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American Society of Microbiology ABMM Sample Questions:

1. A microbiology laboratory is processing a throat swab for the detection of Group A Streptococcus. Culture on sheep blood agar reveals beta-hemolytic colonies. Which of the following tests can be used to presumptively identify Streptococcus pyogenes?

A) Bile esculin hydrolysis
B) Bacitracin susceptibility
C) Catalase test
D) Coagulase test

2. A patient with a history of recurrent urinary tract infections caused by Escherichia coli develops a new infection. The urine isolate is resistant to trimethoprim-sulfamethoxazole. The MOST common mechanism of resistance to this antibiotic combination in E. coli involves:

A) Increased production of para-aminobenzoic acid (PABA).
B) Mutations in the genes encoding dihydrofolate reductase and dihydropteroate synthase.
C) Enzymatic inactivation of trimethoprim and sulfamethoxazole.
D) Expression of efflux pumps that specifically target trimethoprim and sulfamethoxazole.

3. A neonate develops a vesicular rash, fever, and seizures within the first week of life. CSF analysis reveals lymphocytic pleocytosis and elevated protein. PCR of the CSF is positive for a double-stranded DNA virus.
The MOST likely causative agent is:

A) Varicella-zoster virus (VZV)
B) Cytomegalovirus (CMV)
C) Herpes simplex virus (HSV)
D) Human parvovirus B19

4. An elderly patient in a long-term care facility develops a localized outbreak of painful vesicles along a dermatome. Tzanck smear of a vesicle reveals multinucleated giant cells. The MOST likelycausative agent is:

A) Varicella-zoster virus
B) Herpes simplex virus type 1
C) Orf virus
D) Coxsackievirus

5. A patient with a history of travel to a rural area develops a febrile illness with myalgias and thrombocytopenia. Laboratory testing reveals the presence of morulae within circulating leukocytes. The MOST likely diagnosis is:

A) Ehrlichiosis
B) Babesiosis
C) Lyme disease
D) Anaplasmosis

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